AUREMN/CEBEM Course
São Paulo School on Multidimensional NMR in Structural Biology
January 18-23, 2016
Institute of Chemistry of the University of São Paulo
Solution NMR spectroscopy provides high-resolution structural information on proteins and nucleic acids in solution. Additionally, NMR is unique in its power to monitor the internal motions of biological macromolecules at various time scales, and provides a wealth of information on protein interactions, particularly on weak complexes. This first school on "multidimensional NMR in structural biology" will bring young scientists, PhD students and postdocs in contact with the state of the art of the NMR spectroscopy methods applied to biological macromolecules and metabolomics through lectures presented by experts in the field.
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Registration
People interested in attending the course are requested to submit a curriculum (Lattes for Brazilians), a recommendation letter from the immediate supervisor and a letter of interest describing impact/importance of attending the course on their research. Participants are requested to inform whether they will have their own funding for travel/hotel expenses.
The course will be limited to 30 participants!
Please note the dates:
| October 15th, 2015 | Registration will be opened |
| November 15th, 2015 | Registration deadline |
| November 22nd, 2015 | Registration deadline |
Registration Fees (in Brazilian Reais)
| Researchers/Professors AUREMN/CEBEM Members | 450,00 |
| Researchers/Professors Non-members | 1000,00 |
| Pósdoc AUREMN/CEBEM Members | 350,00 |
| Pósdoc Non-members | 800,00 |
| Students AUREMN/CEBEM Members | 250,00 |
| Students Non-members | 600,00 |
Acommodation and travel expenses will be partially funded for selected students (Travel – more than 600 km from SP).
Cancellation Policy
Cancellations will only be accepted in exceptional cases.
How to register
Send all required information and attached letters to
nmrschool@auremn.org.br. You will be notified by email if you are accepted and how to make the payment.
Tentative Program
| Sunday 17/01 |
Arrival |
| Monday 18/01 |
|
| 9:00 - 10:30 |
Lecture |
Product Operator 1 |
CG |
| 10:30 - 11:00 |
Coffee-Break |
| 11:00 - 12:30 |
Lecture |
Product Operator 2 |
CG |
| 12:30 - 14:00 |
Lunch |
| 14:00 - 15:30 |
Lecture |
Introduction to 2D and 3D NMR |
CG |
| 15:30 - 17:00 |
Exercises |
|
CG |
| 17:00 - 17:30 |
Coffee-Break |
| 17:30 - 19:00 |
Exercises |
|
CG |
| Tuesday 19/01 |
|
| 9:00 - 10:30 |
Lecture |
Metabolomics |
RB |
| 10:30 - 11:00 |
Coffee-Break |
| 11:00 - 12:30 |
Lecture |
Metabolomics |
RB |
| 12:30 - 14:00 |
Lunch |
| 14:00 - 15:30 |
Lecture |
Multidimensional heteronuclear NMR pulse sequences |
MS and CG |
| 15:30 - 16:00 |
Coffee-Break |
| 16:00 - 17:30 |
Lecture |
Multidimensional heteronuclear NMR pulse sequences |
MS and CG |
| 17:30 - 18:00 |
Coffee-Break |
| 18:00 - 20:30 |
Exercises |
3D HNCO |
MS and CG |
| Wednesday 20/01 |
|
| 9:00 - 10:30 |
Lecture |
Relaxation 1 (Introduction – qualitative) |
AP |
| 10:30 - 11:00 |
Coffee-Break |
| 11:00 - 12:30 |
Lecture |
Relaxation 2 (Redfield equations) |
AP |
| 12:30 - 14:00 |
Lunch |
| 14:00 - 15:30 |
Lecture |
Relaxation (chemical exchange, T1rho, CPMG) |
AP |
| 15:30 - 16:00 |
Coffee-Break |
| 16:00 - 18:30 |
Tutorial |
Calculation of NMR order parameters |
AP |
| 18:30 - 19:00 |
Coffee-Break |
| 19:00 - 20:30 |
Lecture |
TROSY and cross-correlated relaxation |
CG |
| Thursday 21/01 |
|
| 9:00 - 10:30 |
Lecture |
Partial alignment of biomolecules |
MZ |
| 10:30 - 11:00 |
Coffee-Break |
| 11:00 - 12:30 |
Exercises |
Partial alignment of biomolecules |
MZ |
| 12:30 - 14:00 |
Lunch |
| 14:00 - 15:30 |
Lecture |
Computation1: structure calculation |
MN |
| 15:30 - 15:45 |
Coffee-Break |
| 15:45 - 17:30 |
Lecture |
Computation1: structure calculation |
MN |
| 17:30 - 19:00 |
Lecture |
Computation2: calculation of NMR parameters (CS and RDC averaging, PPM) |
RB |
| 19:00 - 19:30 |
Coffee-Break |
| 19:30 - 21:00 |
Lecture |
Computation2: calculation of NMR parameters (CS and RDC averaging, PPM) |
RB |
| Friday 22/01 |
|
| 9:00 - 10:30 |
Lecture |
Paramagnetic NMR |
CG, MS, MZ |
| 10:30 - 11:00 |
Coffee-Break |
| 11:00 - 12:30 |
Exercises |
Paramagnetic NMR |
CG, MS, MZ |
| 12:30 - 14:00 |
Lunch |
| 14:00 - 15:30 |
Lecture |
The CCPNMR model |
GV |
| 15:30 - 15:45 |
Coffee-Break |
| 15:45 - 16:30 |
Lecture |
The CCPNMR model |
GV |
| 16:30 - 18:00 |
Lecture |
Non-uniform sampling |
HA |
| 18:00 - 20:00 |
Coffee-Break and poster session |
| Saturday 23/01 |
|
| 9:00 - 10:30 |
Tutorial |
Non-uniform sampling |
HA |
| 10:30 - 11:00 |
Coffee-Break |
| 11:00 - 12:30 |
Lecture |
Bruker |
|
| 12:30 - 14:00 |
Lunch |
| 14:00 - 15:30 |
Lecture |
Bruker |
|
| 15:30 - 15:45 |
Coffee-Break |
| 15:45 - 18:80 |
Lecture |
Bruker |
|
CG = Christian Griesinger; MS = Michael Sattler; MZ = Markus Zweckstetter; GV = GeertenVuister; AP = Arthur Palmer; RB = Rafael Brüschweiler; MN = Michael Nilges; HA = Haribabu Arthanari
All instructors should help with the exercises; students will be asked to bring their laptops for the tutorial sessions